Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3A88531" target="_blank" >RIV/60460709:41210/22:88531 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.scitotenv.2021.151783" target="_blank" >https://doi.org/10.1016/j.scitotenv.2021.151783</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.scitotenv.2021.151783" target="_blank" >10.1016/j.scitotenv.2021.151783</a>

Result language
angličtina
Original language name
Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods
Original language description
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for identification of whole communities within a targeted group) is revolutionizing the field of aquatic biomonitoring. To date, most metabarcoding studies aiming to assess the ecological status of aquatic ecosystems have focused on water eDNA and macroinvertebrate bulk samples. However, the eDNA metabarcoding has also been applied to soft sediment samples, mainly for assessing microbial or meiofaunal biota. Compared to classical methodologies based on manual sorting and morphological identification of benthic taxa, eDNA metabarcoding offers potentially important advantages for assessing the environmental quality of sediments. The methods and protocols utilized for sediment eDNA metabarcoding can vary considerably among studies, and standardization efforts are needed to improve their robustness, comparability and use within regulatory frameworks. Here, we review the available information on eDNA metabarcoding applied to
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)

Project
<a href="/en/project/EF16_019%2F0000845" target="_blank" >EF16_019/0000845: Centre for investigation of synthesis and transformation of nutritional substances in the food chain in interaction with potentially harmful substances of athropogenic origin: assessment of contamination risks for the quality of production</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)