Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A94542" target="_blank" >RIV/60460709:41210/23:94542 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2076-2615/13/7/1200" target="_blank" >https://www.mdpi.com/2076-2615/13/7/1200</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ani13071200" target="_blank" >10.3390/ani13071200</a>

Result language
angličtina
Original language name
Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm
Original language description
This study investigated the effect of cryoprotectant glycerol addition in different phases of sperm equilibration on sperm motility and viability parameters of thawed ram spermatozoa. The purpose of this study was to compare two strategies of glycerol addition for sperm cryo-preservation. The first strategy included the use of a glycerol-free extender for the procedure of glycerol-free equilibration and chilling, with the glycerolation of the extender by 6% glycerol shortly before sperm slow freezing. The second strategy included the use of a freezing extender already glycerolated by 6% glycerol before the equilibration and chilling of sperm and following slow freezing. The obtained results were statistically significant and demonstrated that the glycerol-free equilibration with the addition of glycerol at least 10 min before freezing is the best strategy for cryopreservation of Wallachian ram sperm. This represents a perspective strategy in the cryopreservation process for local sheep breeds and indicates a potential strategy to test for ram cryopreservation in a generalized manner.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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