Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021–2023
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A96949" target="_blank" >RIV/60460709:41210/23:96949 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.17221/152/2023-CJAS" target="_blank" >https://doi.org/10.17221/152/2023-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/152/2023-CJAS" target="_blank" >10.17221/152/2023-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021–2023
Original language description
Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
<a href="/en/project/QK22010270" target="_blank" >QK22010270: Management optimalization of individual reproduction performance in dairy cattle</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1212-1819
Volume of the periodical
68
Issue of the periodical within the volume
12
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
17
Pages from-to
469-485
UT code for WoS article
001131885100004
EID of the result in the Scopus database
2-s2.0-85181500788