Antimicrobial peptides and proteins as alternative antibiotics for porcine semen preservation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F24%3A101477" target="_blank" >RIV/60460709:41210/24:101477 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1186/s12917-024-04105-9" target="_blank" >https://doi.org/10.1186/s12917-024-04105-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12917-024-04105-9" target="_blank" >10.1186/s12917-024-04105-9</a>
Alternative languages
Result language
angličtina
Original language name
Antimicrobial peptides and proteins as alternative antibiotics for porcine semen preservation
Original language description
Background Antimicrobial resistance (AMR) is nowadays a major emerging challenge for public health worldwide. The over- and misuse of antibiotics, including those for cell culture, are promoting AMR while also encouraging the research and employment of alternative drugs. The addition of antibiotics to the cell media is strongly recommended in sperm preservation, being gentamicin the most used for boar semen. Because of its continued use, several bacterial strains present in boar semen have developed resistance to this antibiotic. Antimicrobial peptides and proteins (AMPPs) are promising candidates as alternative antibiotics because their mechanism of action is less likely to promote AMR. In the present study, we tested two AMPPs (lysozyme and nisin; 50 and 500 mu g/mL) as possible substitutes of gentamicin for boar semen preservation up to 48 h of storage. Results We found that both AMPPs improved sperm plasma membrane and acrosome integrity during semen storage. The highest concentration tested for lysozyme also kept the remaining sperm parameters unaltered, at 48 h of semen storage, and reduced the bacterial load at comparable levels of the samples supplemented with gentamicin (p > 0.05). On the other hand, while nisin (500 mu g/mL) reduced the total Enterobacteriaceae counts, it also decreased the rapid and progressive sperm population and the seminal oxidation-reduction potential (p < 0.05). Conclusions The protective effect of lysozyme on sperm function together with its antimicrobial activity and inborn presence in body fluids, including semen and cervical mucus, makes this enzyme a promising antimicrobial agent for boar semen preservation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Veterinary Research
ISSN
1746-6148
e-ISSN
1746-6148
Volume of the periodical
20
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
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UT code for WoS article
001246055300004
EID of the result in the Scopus database
2-s2.0-85195948292