Effects of age and litter-of-origin on cryopreserved spermatozoa in Sumava rams
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F24%3A98588" target="_blank" >RIV/60460709:41210/24:98588 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.17221/32/2024-CJAS" target="_blank" >https://doi.org/10.17221/32/2024-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/32/2024-CJAS" target="_blank" >10.17221/32/2024-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Effects of age and litter-of-origin on cryopreserved spermatozoa in Sumava rams
Original language description
This study aimed to evaluate the influence of the internal factors of ram age and litter-of-origin on semen quality during the cryopreservation process in Sumava sheep rams. This breed is included in the protected genetic resources of the Czech Republic. The sires were systematically divided into four groups according to age (under 1.5 years, 1.5 to 2.5 years, 2.5 to 3.5 years, and over 4.5 years) and according to litter frequency (singletons vs twins). Semen was evaluated after equilibration, and after cryopreservation using iSperm (R) mCASA and flow cytometry. During cryopreservation, there was a significant decrease in total sperm motility by 53.5%, progressive motility by 38%, and cells with intact plasma membrane and acrosome by 47%. Frozen-thawed sperm kinematic parameters showed significant age-related variations, with rams aged 4.5 and older displaying notably higher total and progressive motility (16.2% and 6.24%, respectively). Rams born as twins exhibited 3.77% (P < 0.05) higher progressive motility and 5.5% (P < 0.05) higher total motility compared to those born as singles. The sperm of older rams (> 4.5 years) exhibited higher viability (10.1%) and lower damage to the plasma membrane after freeze-thawing (23.6%), (P < 0.05). Twins showed significantly higher sperm viability (4.98%, P < 0.05) than singletons. These rams produce a larger quantity of higher-quality insemination doses after cryopreservation. For Sumava rams, in particular, broadening the sire selection base helps to select suitable rams for breeding. As the sire ages, his genetic value within the production herd may decline with time. This contrasts with genetic resource protection, where the aim is to preserve and store as many high-quality semen samples as possible.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1805-9309
Volume of the periodical
69
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
129-138
UT code for WoS article
001218559100001
EID of the result in the Scopus database
2-s2.0-85192970383