A novel <i>Erwiniaceae</i> gut symbiont modulates gene expression of the intracellular bacterium <i>Cardinium</i> in the stored product mite <i>Tyrophagus putrescentiae</i>
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102356" target="_blank" >RIV/60460709:41210/25:102356 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1128/msphere.00879-24" target="_blank" >https://doi.org/10.1128/msphere.00879-24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/msphere.00879-24" target="_blank" >10.1128/msphere.00879-24</a>
Alternative languages
Result language
angličtina
Original language name
A novel <i>Erwiniaceae</i> gut symbiont modulates gene expression of the intracellular bacterium <i>Cardinium</i> in the stored product mite <i>Tyrophagus putrescentiae</i>
Original language description
We examined host and bacterial gene expression profiles in the stored product mite Tyrophagus putrescentiae co-infected with Wolbachia (wTPut) and Cardinium (cTPut) while varying the presence of the Erwiniaceae symbiont (SLS). SLS, a novel symbiont in the family Erwiniaceae, with a genome size of 1.7 Mb, is found in 16% of mite species in infected cultures. In addition, SLS was detected in mite feces but not in their eggs. Although Wolbachia expression remained unchanged, the presence or absence of SLS significantly affected Cardinium expression. It indicated that the effect of Wolbachia on SLS was neutral. In SLS-positive samples, Cardinium exhibi ted 29 upregulated and 48 downregulated genes compared to SLS-negative samples. Furthermore, Cardinium gene expression strongly correlated with mite KEGG gene expression in SLS-positive samples. Positive Spearman's correlations between Cardinium gene expression and mite KEGG immune and regulatory pathways were doubled in SLS-positive compared to SLS-negative samples. The diversity of expressed genes in the mite host decreased in the presence of SLS. Cardinium had more interacting genes to mite host in SLS-positive samples than without SLS. Transposases are the most affected Cardinium genes, showing upregulation in the presence of SLS. Correlation analyses revealed interactions between Cardinium and SLS via mite immune and regulatory pathways, including lysosome, ubiquitin-mediated proteolysis, PIK3_Akt, and cGMP-PKG. The results showed that Cardinium indirectly affects the gut symbionts of mites.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
mSphere
ISSN
2379-5042
e-ISSN
2379-5042
Volume of the periodical
10
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
26
Pages from-to
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UT code for WoS article
001450428500001
EID of the result in the Scopus database
2-s2.0-105004234407