Comparative evaluation of an AI-based counting system (OvaCyteTM) and the McMaster counting method for quantification of strongyle eggs in sheep faeces
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102723" target="_blank" >RIV/60460709:41210/25:102723 - isvavai.cz</a>
Result on the web
<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:001520072200001" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:001520072200001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2025.110533" target="_blank" >10.1016/j.vetpar.2025.110533</a>
Alternative languages
Result language
angličtina
Original language name
Comparative evaluation of an AI-based counting system (OvaCyteTM) and the McMaster counting method for quantification of strongyle eggs in sheep faeces
Original language description
We compared an artificial intelligence (AI)-based technology (OvaCyte™, OC) for the enumeration of strongyle eggs in sheep faeces with the McMaster method (MM). Initially, two experiments were performed with faeces containing pure Haemonchus contortus eggs. In experiment A, faeces containing three egg concentrations were processed using OC (extended and standard mode) in parallel with MM. In experiment B, faeces were spiked with different amounts of eggs. Secondly, samples from naturally infected sheep were analysed. Overall, EPG values in experiment A were consistent across all replicates at each dilution. Accuracy was particularly good for the AI-method (mean OC=72 %, mean MM=45 %), and it also achieved the highest precision (CV 5.6–40 %). In experiment B, as in experiment A, within replicate variability was observed at for both methods all concentrations. Although there were no significant differences between sample means, precision and the number of egg-positive samples was higher for OC. Finally, analysis of both experimental (r = 0.98) and field samples (r = 0.93) showed a strong positive correlation between OC and MM. OC also yielded a higher proportion of positive samples than MM in the field study OC provided a higher proportion of positive samples than MM. This study is the first comparison of OC and MM using both experimental and field-based data. In contrast to previous studies, our analysis was based on identical sample preparations that were processed in parallel using both methods. Although the results show strong agreement between methods, some limitations of OC were noted. These limitations can probably be overcome by further refinement of the AI model.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Veterinary Parasitology
ISSN
0304-4017
e-ISSN
1873-2550
Volume of the periodical
338
Issue of the periodical within the volume
neuvedeno
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1-8
UT code for WoS article
001520072200001
EID of the result in the Scopus database
2-s2.0-105008732261