Molecular Identification of Zoonotic Taeniids Metacestodes in Several Rodent Species Trapped in Central Europe

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102898" target="_blank" >RIV/60460709:41210/25:102898 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1571082/full" target="_blank" >https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1571082/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2025.1571082" target="_blank" >10.3389/fvets.2025.1571082</a>

Result language

angličtina

Original language name

Molecular Identification of Zoonotic Taeniids Metacestodes in Several Rodent Species Trapped in Central Europe

Original language description

Background Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that morphology alone may not provide accurate identification. In this study, rodent livers from various habitats across five regions of the Czech Republic were examined for the presence of taeniid larval cestodes.Methods A total of 2,934 rodent specimens were collected using snap traps between 2014 and 2022. Taeniid metacestodes detected in these intermediate hosts were subsequently subjected to species determination through molecular (targeting the cytochrome oxidase subunit I gene) and morphological examinations.Results The following cestodes were identified using molecular methods: Hydatigera kamiyai (found in Apodemus flavicollis, Apodemus sylvaticus, Microtus arvalis, and Microtus agrestis), Taenia martis (in A. flavicollis, M. arvalis, and Myodes glareolus), and Versteria mustelae (in M. arvalis and My. glareolus). Sequencing of 55 PCR-positive isolates revealed 13 haplotypes of H. kamiyai, one of T. martis and four of V. mustelae. Further, we also identified co-infection by more metacestode species or haplotypes. No rodent liver samples tested were positive for Echinococcus multilocularis or Hydatigera taeniaeformis sensu stricto, either through morphological or molecular identification methods. Furthermore, no other cryptic species were detected during this study.Conclusion This study presents the first comprehensive molecular data on the aforementioned zoonotic cestode species in the Czech Republic. The findings demonstrate the importance of using molecular techniques for Taeniidae species determination, as morphological methods may lead to erroneous classifications. Furthermore, this research highlights the importance of accurate diagnostic techniques, enabling the development of effective prevention and control strategies by verifying host-parasite relationships.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/GA23-07811S" target="_blank" >GA23-07811S: Interaction of parasites and metal(loids) in small terrestrial mammals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FRONTIERS IN VETERINARY SCIENCE

ISSN

2297-1769

e-ISSN

2297-1769

Volume of the periodical

12

Issue of the periodical within the volume

neuvedeno

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

1-13

UT code for WoS article

001499962000001

EID of the result in the Scopus database

2-s2.0-105006976782