Somatic embryogenesis of the hybrid Abies cilicica * Abies cephalonica

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F11%3A51524" target="_blank" >RIV/60460709:41320/11:51524 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Somatic embryogenesis of the hybrid Abies cilicica * Abies cephalonica

Original language description

The interspecific hybrid fir Abies cilicica * Abies cephalonica is based on native species Abies cephalonica (Loud.) and Abies cilicica (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. The medium BAP at a concentration of 1 mg l?1, 1,000 mg l?1 myo-inositol, 500 mg l?1 glutamine, and 1,000 mg l?1 casein was found to be the most suitable initiation medium for somatic embryogenesis of this hybrid. Embryogenic suspensor mass was produced from immature and mature primary explants. The initiation frequency of ESM was dependent on the composition of nutrient medium, plant growth regulators, and time of seeds collection. The ESM initiation from immature seeds was higher in comparison with mature zygotic embryos, but the results were not significantly different. As the most suitable maturation medium was evaluated the medium based on Murashige and Skoog medium which contained 10 mg l?1 abscisic acid, 100 g l?1 PEG and 40 g l?1 maltose (MM 2 medium).

Czech name

Somatic embryogenesis of the hybrid Abies cilicica * Abies cephalonica

Czech description

The interspecific hybrid fir Abies cilicica * Abies cephalonica is based on native species Abies cephalonica (Loud.) and Abies cilicica (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. The medium BAP at a concentration of 1 mg l?1, 1,000 mg l?1 myo-inositol, 500 mg l?1 glutamine, and 1,000 mg l?1 casein was found to be the most suitable initiation medium for somatic embryogenesis of this hybrid. Embryogenic suspensor mass was produced from immature and mature primary explants. The initiation frequency of ESM was dependent on the composition of nutrient medium, plant growth regulators, and time of seeds collection. The ESM initiation from immature seeds was higher in comparison with mature zygotic embryos, but the results were not significantly different. As the most suitable maturation medium was evaluated the medium based on Murashige and Skoog medium which contained 10 mg l?1 abscisic acid, 100 g l?1 PEG and 40 g l?1 maltose (MM 2 medium).

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GK - Forestry

OECD FORD branch

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Project

<a href="/en/project/ME%20914" target="_blank" >ME 914: Fir Breeding for Forestry and Christmas Tree Production</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Forest Science

ISSN

1212-4834

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

9

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

401-408

UT code for WoS article

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EID of the result in the Scopus database

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