Simplified in vitro propagation protocol for Tacca leontopetaloides (L.) Kuntze and assessment of genetic uniformity of regenerated plantlets
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F15%3A68022" target="_blank" >RIV/60460709:41320/15:68022 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41340/15:68022
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Simplified in vitro propagation protocol for Tacca leontopetaloides (L.) Kuntze and assessment of genetic uniformity of regenerated plantlets
Original language description
Tacca leontopetaloides is a tuberous species with nutritional, medicinal, and industrial potential. The aim of the present study was to develop a simplified micropropagation protocol for this species, combining phases of in vitro propagation and rooting,and to assess the genetic stability of in vitro regenerants using ISSR and flow cytometric analysis. In the first step, the effect of four cytokinins (BA, zeatin, kinetin, thidiazuron) at concentrations of 0.1-0.7 mg l-1 on shoot organogenesis was tested. The highest number of shoots was achieved on MS medium supplemented with 0.1 mg l-1 zeatin (4.11 ? 0.35 shoots/explant). Thereafter, this treatment was combined with auxins (NAA, IAA) at concentrations of 0.01-0.07 mg l-1 to study shoot organogenesisand in vitro rooting. A combination of plant growth regulators provided unchanged (high) shoot organogenesis, while the root number per explant increased. The highest number of roots of all tested treatments was produced on MS medium supp
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Emirates Journal of Food and Agriculture
ISSN
2079-052X
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
10
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
736-743
UT code for WoS article
000368570100002
EID of the result in the Scopus database
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