Cap 'n' collar C regulates genes responsible for imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F18%3A78734" target="_blank" >RIV/60460709:41320/18:78734 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.ibmb.2018.05.006" target="_blank" >http://dx.doi.org/10.1016/j.ibmb.2018.05.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibmb.2018.05.006" target="_blank" >10.1016/j.ibmb.2018.05.006</a>

Result language

angličtina

Original language name

Cap 'n' collar C regulates genes responsible for imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Original language description

The Colorado potato beetle (CPB), Leptinotarsa decemlineata developed resistance to imidacloprid after exposure to this insecticide for multiple generations. Our previous studies showed that xenobiotic transcription factor, cap n collar isoform C (CncC) regulates the expression of multiple cytochrome P450 genes, which play essential roles in resistance to plant allelochemicals and insecticides. In this study, we sought to obtain a comprehensive picture of the genes regulated by CncC in imidacloprid-resistant CPB. We performed sequencing of RNA isolated from imidacloprid-resistant CPB treated with dsRNA targeting CncC or gene coding for green fluorescent protein (control). Comparative transcriptome analysis showed that CncC regulated the expression of 1798 genes, out of which 1499 genes were downregulated in CncC knockdown beetles. Interestingly, expression of 79% of imidacloprid induced P450 genes requires CncC. We performed quantitative real-time PCR to verify the reduction in the expression of 20 g

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

ISSN

0965-1748

e-ISSN

—

Volume of the periodical

99

Issue of the periodical within the volume

AUG2018

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

54-62

UT code for WoS article

000436915100006

EID of the result in the Scopus database

2-s2.0-85048191739