Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F19%3A81176" target="_blank" >RIV/60460709:41320/19:81176 - isvavai.cz</a>
Result on the web
<a href="https://www.pnas.org/content/116/31/15677" target="_blank" >https://www.pnas.org/content/116/31/15677</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1902089116" target="_blank" >10.1073/pnas.1902089116</a>
Alternative languages
Result language
angličtina
Original language name
Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta
Original language description
The hawkmoth Manduca sexta and one of its preferred hosts in the North American Southwest, Datura wrightii, share a model insect-plant relationship based on mutualistic and antagonistic life-history traits. D. wrightii is the innately preferred nectar source and oviposition host for M. sexta. Hence, the hawkmoth is an important pollinator while the M. sexta larvae are specialized herbivores of the plant. Olfactory detection of plant volatiles plays a crucial role in the behavior of the hawkmoth. In vivo, the odorant receptor coreceptor (Orco) is an obligatory component for the function of odorant receptors (ORs), a major receptor family involved in insect olfaction. We used CRISPR-Cas9 targeted mutagenesis to knock out (KO) the MsexOrco gene to test the consequences of a loss of OR-mediated olfaction in an insect-plant relationship. Neurophysiological characterization revealed severely reduced antennal and antennal lobe responses to representative odorants emitted by D. wrightii. In a wind-tunnel set
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10614 - Behavioral sciences biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the national Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
116
Issue of the periodical within the volume
31
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
15677-15685
UT code for WoS article
000477812400055
EID of the result in the Scopus database
2-s2.0-85070789692