Robust reference gene selection in Norway spruce: essential for real-time quantitative PCR across different tissue, stress and developmental conditions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F24%3A100348" target="_blank" >RIV/60460709:41320/24:100348 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/24:43925976
Result on the web
<a href="http://dx.doi.org/10.3389/ffgc.2024.1458554" target="_blank" >http://dx.doi.org/10.3389/ffgc.2024.1458554</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/ffgc.2024.1458554" target="_blank" >10.3389/ffgc.2024.1458554</a>
Alternative languages
Result language
angličtina
Original language name
Robust reference gene selection in Norway spruce: essential for real-time quantitative PCR across different tissue, stress and developmental conditions
Original language description
Accurate gene expression analysis in Norway spruce (Picea abies) under diverse stress conditions requires the identification of stable reference genes for normalization. Notably, the literature lacks reports on suitable reference genes in Norway spruce. Here, we aimed to address this gap by identifying suitable reference genes for quantitative real-time PCR in Norway spruce across various stress conditions (drought, heat, pathogen infection) in seedlings, tissues (needle, phloem, root), and developmental stages (seedlings, mature trees). We evaluated the stability of 15 candidate reference genes and assessed their expression stability using five statistical algorithms (Delta Ct, geNorm, NormFinder, BestKeeper, and RefFinder). Our results highlight ubiquitin-protein ligase (SP1), conserved oligomeric Golgi complex (COG7), and tubby-like F-box protein (TULP6) as the most stable reference genes, while succinate dehydrogenase (SDH5) and heat shock protein 90 (HSP90) were the least stable under various experimental conditions. COG7 and TULP6 are novel candidate reference genes reported for the first time. The expression stability of the identified reference genes was further validated using dehydrin-like protein 5 (PaDhn5) under drought conditions in Norway spruce. Pairwise variation analysis suggests that two reference genes were sufficient to normalize gene expression across all sample sets. This study provides a comprehensive analysis of reference gene stability under different experimental conditions and a catalog of genes for each condition, facilitating future functional genomic research in Norway spruce and related conifers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40100 - Agriculture, Forestry, and Fisheries
Result continuities
Project
<a href="/en/project/LTT20017" target="_blank" >LTT20017: Participation of the Czech Republic in the network of experiments TreeDivNet</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FRONTIERS IN FORESTS AND GLOBAL CHANGE
ISSN
2624-893X
e-ISSN
2624-893X
Volume of the periodical
7
Issue of the periodical within the volume
17.0
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
1-17
UT code for WoS article
001363697500001
EID of the result in the Scopus database
2-s2.0-85210521749