Gene expression plasticity facilitates different host feeding in Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F24%3A100565" target="_blank" >RIV/60460709:41320/24:100565 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.ibmb.2023.104061" target="_blank" >http://dx.doi.org/10.1016/j.ibmb.2023.104061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibmb.2023.104061" target="_blank" >10.1016/j.ibmb.2023.104061</a>

Result language

angličtina

Original language name

Gene expression plasticity facilitates different host feeding in Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae)

Original language description

Host shift is ecologically advantageous and a crucial driver for herbivore insect speciation. Insects on the nonnative host obtain enemy -free space and confront reduced competition, but they must adapt to survive. Such signatures of adaptations can often be detected at the gene expression level. It is astonishing how bark beetles cope with distinct chemical environments while feeding on various conifers. Hence, we aim to disentangle the six -toothed bark beetle (Ips sexdentatus) response against two different conifer defences upon host shift (Scots pine to Norway spruce). We conducted bioassay and metabolomic analysis followed by RNA-seq experiments to comprehend the beetle's ability to surpass two different terpene-based conifer defence systems. Beetle growth rate and fecundity were increased when reared exclusively on spruce logs (alternative host) compared to pine logs (native host). Comparative gene expression analysis identified differentially expressed genes (DEGs) related to digestion, detoxification, transporter activity, growth, signalling, and stress response in the spruce -feeding beetle gut. Transporter genes were highly abundant during spruce feeding, suggesting they could play a role in pumping a wide variety of endogenous and xenobiotic compounds or allelochemicals out. Trehalose transporter (TRET) is also up -regulated in the spruce -fed beetle gut to maintain homeostasis and stress tolerance. RTqPCR and enzymatic assays further corroborated some of our findings. Taken together, the transcriptional plasticity of key physiological genes plays a crucial role after the host shift and provides vital clues for the adaptive potential of bark beetles on different conifer hosts.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

ISSN

0965-1748

e-ISSN

0965-1748

Volume of the periodical

165

Issue of the periodical within the volume

16.0

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

16

Pages from-to

1-16

UT code for WoS article

001151970900001

EID of the result in the Scopus database

2-s2.0-85181812083