Comprehensive Metabolomic Profiling and Biological Activity Analysis of Eschscholzia californica Extracts Using LC-ESI-QTOF-MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F25%3A102250" target="_blank" >RIV/60460709:41320/25:102250 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/fsn3.70885" target="_blank" >https://doi.org/10.1002/fsn3.70885</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/fsn3.70885" target="_blank" >10.1002/fsn3.70885</a>
Alternative languages
Result language
angličtina
Original language name
Comprehensive Metabolomic Profiling and Biological Activity Analysis of Eschscholzia californica Extracts Using LC-ESI-QTOF-MS
Original language description
The current investigation was designed to explore the chemical composition, antioxidant capacity, enzyme inhibitory activity, and cytotoxic potential of four different extracts (Ethyl Acetate, Ethanol, Ethanol/Water (70%) and Water) derived from the aerial parts of Eschscholzia californica. In vitro, assessments were performed utilizing diverse antioxidant assays, along with evaluations of neuroprotective enzyme inhibition targeting acetylcholine and butyl choline enzymes, as well as antidiabetic activities against alpha-amylase and alpha-glucosidase and a potential candidate for a tyrosinase inhibitor. LC-ESI-QTOF-MS identification provided a total of 70 compounds in the extracted samples of E. californica, including kaempferol 3-(deoxyhexosyl-hexoside)-7-hexoside, rutin, quercetin dideoxyhexoside, caffeic acid hexoside, quinoline alkaloids, morphine derivatives, and scoulerine. Moreover, the ethanol extract exhibited the highest anti-AChE (2.39 mg GALAE/g), while ethyl acetate exhibited anti-BChE (3.31 mg GALAE/g), Ethanol/Water (70%) anti-tyrosinase (53.09 mg KAE/g) and anti-glucosidase (1.09 mmol ACAE/g) activities. Additionally, the ethyl acetate extract effectively inhibited carbonic anhydrase I and II isoenzymes. Furthermore, the ethanol and ethanol/water extracts demonstrated significant cytotoxicity against A549 lung cancer cells. However, E. californica partially/weakly triggers the apoptosis of cancer cells. Furthermore, the investigation identified 885 target genes for E. californica's phytochemicals, 31 of which were familiar to insomnia. In silico studies demonstrated that protopine, rutin, eschscholtzidine, boldine, and (S)-scoulerine exhibited notable inhibitory effects on insomnia-related DRD5, DRD4, and SERT proteins. These findings highlight the potential pharmacological applications of the aerial parts of E. californica as a source for developing novel phytopharmaceuticals targeting various oxidative stress-related conditions, including diabetes, cancer, Alzheimer's disease, and insomnia.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40401 - Agricultural biotechnology and food biotechnology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FOOD SCIENCE & NUTRITION
ISSN
2048-7177
e-ISSN
2048-7177
Volume of the periodical
13
Issue of the periodical within the volume
SEP 2025
Country of publishing house
GB - UNITED KINGDOM
Number of pages
20
Pages from-to
1-20
UT code for WoS article
001580659200005
EID of the result in the Scopus database
2-s2.0-105014876892