Not All Bark Beetles Smell the Same: Population-Level Functional Olfactory Polymorphisms in Ips typographus Pheromone Receptor ItypOR33

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F25%3A102972" target="_blank" >RIV/60460709:41320/25:102972 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/mec.17693" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/mec.17693</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/mec.17693" target="_blank" >10.1111/mec.17693</a>

Result language

angličtina

Original language name

Not All Bark Beetles Smell the Same: Population-Level Functional Olfactory Polymorphisms in Ips typographus Pheromone Receptor ItypOR33

Original language description

Eurasian spruce bark beetle Ips typographus, a natural part of forest ecosystems, is a major threat to Norway spruce forests during outbreaks. Olfaction plays a crucial role in the survival and range expansion of these beetles, amid forest disturbances and climate change. As the current management strategies are suboptimal for controlling outbreaks, the reverse chemical ecology approaches based on pheromone receptors offer promising alternatives. While the search for pheromone receptors is in progress, recently found chromosomal inversions indicates signs of adaptation in this species. Our attempts to characterise one of the highly expressed odorant receptors, ItypOR33, located in an inversion, led to the discovery of polymorphic variants distributed with similar frequency across 18 European populations. Deorphanizing ItypOR33 and its variant ItypOR33a using the Drosophila empty-neuron system (DeNS) revealed ItypOR33 tuned to amitinol, a heterospecific pheromone component in Ips spp., whereas its variant tuned to (S)-(-)-ipsenol, a conspecific pheromone component of I. typographus. The in silico approaches revealed the structural basis of variations by predicting putative ligand-binding sites, tunnels and ligand-receptor interactions. However, no sex-specific differences were found in the ItypOR33 expression, and its ligand amitinol elicited behavioural and electrophysiological responses. Reporting population-level functional olfactory polymorphisms for the first time in a non-model organism-bark beetles, provides key evidence for further exploring their survival and adaptation in forests. Additionally, these findings indicate potential long-term complexities of managing bark beetles in forests.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10618 - Ecology

Project

<a href="/en/project/EF15_003%2F0000433" target="_blank" >EF15_003/0000433: Building up an excellent scientific team and its spatio-technical background focused on mitigation of the impact of climatic changes to forests from the level of a gene to the level of a landscape at the FFWS CULS Prague</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Ecology

ISSN

0962-1083

e-ISSN

0962-1083

Volume of the periodical

34

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

19

Pages from-to

1-19

UT code for WoS article

001427500300001

EID of the result in the Scopus database

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