RNA-sequencing attest increased sperm motility in bovine spermatozoa treated with ethanolic extract of <i>Putranjiva roxburghii</i>
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F23%3A97695" target="_blank" >RIV/60460709:41340/23:97695 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s13205-022-03452-4" target="_blank" >https://doi.org/10.1007/s13205-022-03452-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13205-022-03452-4" target="_blank" >10.1007/s13205-022-03452-4</a>
Alternative languages
Result language
angličtina
Original language name
RNA-sequencing attest increased sperm motility in bovine spermatozoa treated with ethanolic extract of <i>Putranjiva roxburghii</i>
Original language description
In the course of time, scientific communities have a growing interest in understanding ethano medicines. The Putranjiva roxburghii, a native plant of the Indian Subcontinent is described as a "Child amulet tree" in Ayurveda. Based on the fact that this herbal medicine has an indispensable component of integrative medicine, the present study was planned to assess the effect of ethanolic dried extract of Putranjiva seeds on the motility of X and Y-bear ing bovine spermatozoa. The in-vitro effect of seed extract diluted in S-TALP medium on bull semen has been evaluated by Computer Assisted Semen Analysis (CASA) shows a marked increase in the motility of spermatozoa. Motile and non-motile spermatozoa have been separated by glass wool column from the control as well as treated group. The X and Y-bearing sperm quantification have been carried out by droplet digital polymerase chain reaction (ddPCR). The extract didn't exert any differential effect on the motility and viability of X and Y chromosome-bearing spermatozoa. The transcriptome profiling (RNA-Seq) identified 93 differentially expressed genes between the extract treated and control group. It unveils the up-regulation of CATSPER, AKAP3, SPAG, ADAM1B, ADAM2 and ADAM32 genes that are involved in increasing sperm motility. Transcriptome profile also unveil the expression of ZAR1, CYP17A1, APPL2, HOXB4 and SP9 genes involved with embryonic development processes in Putranjiva extract-treated motile spermatozoa. The results envisaged the medicinal value of Putranjiva herb on increased fertility due to combinatory effect like increased sperm motility and favourableness on embryogenesis. The study ruled out the possibility of herbs having any biased effect on the selection of either male or female-bear ing spermatozoa in the bull.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
3 Biotech
ISSN
2190-5738
e-ISSN
2190-5738
Volume of the periodical
13
Issue of the periodical within the volume
JAN 2023
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
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UT code for WoS article
000907114400002
EID of the result in the Scopus database
2-s2.0-85145557832