Sustainable plantlet regeneration from hypocotyl segments and zygotic embryo of Securidaca longipedunculata Fresen. (Polygonaceae) - An endangered medicinal plant

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F23%3A97788" target="_blank" >RIV/60460709:41340/23:97788 - isvavai.cz</a>

Result on the web

<a href="https://pakbs.org/pjbot/paper_details.php?id=10820" target="_blank" >https://pakbs.org/pjbot/paper_details.php?id=10820</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.30848/PJB2023-4(5)" target="_blank" >10.30848/PJB2023-4(5)</a>

Result language

angličtina

Original language name

Sustainable plantlet regeneration from hypocotyl segments and zygotic embryo of Securidaca longipedunculata Fresen. (Polygonaceae) - An endangered medicinal plant

Original language description

Plant regeneration was achieved by culturing sterile hypocotyl segments, and mature zygotic embryos (MZE) of S. longipedunculata Fresen excised from sterile seeds. In this study, de-coated and sterilized seeds were first grown into seedlings In vitro, disjointed into root, hypocotyl, and leaf, segments. The hypocotyl segments (0.5cm) were cultured in root induction media containing MS basal medium + sucrose (30 g l-1) fortified with 0-5 mg l-1 NAA and 0-5 mg l-1 IBA either alone or in combination to produce roots. Rooted hypocotyl segments were cultured in shoot induction media containing 0-1mg l-1 IAA, 0-1 mg l-1 KIN, and 0-1 mg l-1 GA for shoot induction. Second, mature zygotic embryos (MZEs) excised from seeds were cultured based on 3x4x5 factorial - Control, MS and B5 basal media, three carbon sources (sucrose, glucose, and fructose), and control at five concentrations (0-4%) in a completely randomized design at p <= 0.05 and repeated two independent times for reproducibility of results. Results of the observed parameters revealed that after 45 days, the highest number of roots (11.66 +/- 0.23), most increased root length (3.43 +/- 0.23), and highest percent root generation (86.66 +/- 3.33) were produced in hypocotyl explants in 0 mg l-1 NAA+5.0 mg l-1 IBA. More so, the earliest induction (35.66 +/- 0.66) was seen in 0.5 mg l-1 NAA+0.5 mg l-1 IBA. After 72 days of culture, the highest number of new shoots (2.0 per explant), the highest number of leaves (5.0 +/- 1.00), the highest number of shoots (3.80 +/- 0.11), highest percent regeneration (88.33 +/- 1.60), and highest leaf area (2.06 +/- 0.23) were produced in rooted hypocotyl explants at 1mg l-1 each for IAA, KIN, and GA relative to other treatments. In MZE culture, 3% sucrose in MS medium was the most preferred for zygotic embryo culture and differed significantly from other treatments at p <= 0.05. These young plantlets, In vitro, were hardened and raised in the field. These regenerated plantlets would ensure the conservation of this endangered plant as a primary source of medicine.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40101 - Agriculture

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PAKISTAN JOURNAL OF BOTANY

ISSN

0556-3321

e-ISSN

0556-3321

Volume of the periodical

55

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

1375-1382

UT code for WoS article

000972097200018

EID of the result in the Scopus database

2-s2.0-85158903219