Differentiation of Cereal Eyespot Fungi using Morphologic Characteristics and PCR Diagnostic Tests

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A_____%2F00%3A00001517" target="_blank" >RIV/60460709:_____/00:00001517 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/00:00001517

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Differentiation of Cereal Eyespot Fungi using Morphologic Characteristics and PCR Diagnostic Tests

Original language description

Stem parts of winter wheat infected with eyespot caused by Pseudocercosporella herpotrichoides were collected at various locations in the Czech Republic in 1995 - 1998. Pure colonies of the fungi were cultivated on potato-dextrose agar, visually, classified into the teleomorphs and tested for sensitivity to MBC-fungicides. Selected isolates of Tapesia yallundae and T. acuformis were examined using RAPD markers. Of the total 1014 isolates collected, 70% were assessed as W-type (T. yallundae) and 30% as R-type (T. acuformis). The ferquencies of T. yallundae / T. acuformis in each of the 4 years were 73/27, 78/22, 63/65 and 66/34%. That the isolates belonged to different pathotypes was proved by RAPD analysis on ten isolates that had been visually identified as the W-type and on ten of the R-type. Among five tested primers, only 5' GATGACCG 3' provided polymorphic RAPD products at minimum non-specific amplification. The genetic similarity was assessed by Dice's coefficient of similarity w

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F97%2F0636" target="_blank" >GA522/97/0636: Detection and identefitication of the eyespot causal agent, Pseudocercosporella herpotrichoides, using polymerase chain reaction methods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Protection Science

ISSN

1212-2580

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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