Determination of N-methylcarbamoylvaline; globin adducts; metabolism of N,N-dimethylformamide; biological monitoring;

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F02%3A00006523" target="_blank" >RIV/60461373:22310/02:00006523 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Determination of N-methylcarbamoylvaline; globin adducts; metabolism of N,N-dimethylformamide; biological monitoring;

Original language description

A sensitive method for the determination of the N-methylcarbamoyl adduct at the N-terminal valine of globin, a new metabolic product of the industrial solvent N,N-dimethylformamide (DMF), has been developed and validated. The method includes conversion of the adduct by Edman degradation to 3-methyl-5-isopropylhydantoin (MVH). The limit of detection is 0.2 nmol MVH/g globin when a 100-mg sample is used. Within- and between-day precision is 4-10%. The method has been used to determine the background levels of the adduct in unexposed subjects. Further, toxicokinetic studies in volunteers laid the grounds for setting the reference value for biological monitoring of occupational exposure to DMF.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B

ISSN

0021-9673

e-ISSN

—

Volume of the periodical

778

Issue of the periodical within the volume

N

Country of publishing house

BE - BELGIUM

Number of pages

8

Pages from-to

357-365

UT code for WoS article

—

EID of the result in the Scopus database

—