Assessment of in vitro testing approaches for bioactive inorganic materials

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F16%3A43900419" target="_blank" >RIV/60461373:22310/16:43900419 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jnoncrysol.2015.03.016" target="_blank" >http://dx.doi.org/10.1016/j.jnoncrysol.2015.03.016</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jnoncrysol.2015.03.016" target="_blank" >10.1016/j.jnoncrysol.2015.03.016</a>

Result language

angličtina

Original language name

Assessment of in vitro testing approaches for bioactive inorganic materials

Original language description

This paper deals with non-standard in vitro testing of bioactive inorganic materials shaped as granules or scaffolds. The ISO 23317 standard describes the in vitro test arrangement of bulk bioactive materials under static conditions. However, this norm has not dealt with bioactive materials shaped as granules (with large surface area) that are commonly used in clinical practice. We found that in the case of highly reactive (bioactive) materials, the biogenic elements were exhausted from simulated body fluid (SBF) solution very quickly (within hours) under static conditions. In such exhausted SBF solution the formation of Ca-P layer (hydroxyapatite - HAp) was stopped in agreement with the decrease of Ca and P concentration. On the contrary, highly soluble materials (glass-ceramic scaffold) induced the formation of a new mineral layer also on the walls of the PE container used. For a non-standard shape of the tested materials the usage of dynamic or static-dynamic in vitro test arrangement was confirmed to be a better option to test bioactivity. However, also for this type of arrangement it is essential to determine the S/V or S/F ratios (the surface area /volume or flow of SBF solution) very precisely. For detailed understanding of the interaction between the tested material and SBF it is important to analyze the leachates (monitoring Ca2+, (PO4)3- and minor elements concentrations) and to monitor the pH value. An expected result of the in vitro test (according ISO standard) is the formation of HAp on the surfaces of tested samples in SBF. However, the formation of hydroxyapatite may not be the proof of their potential bioactivity necessarily (e.g. due to the use of TRIS buffer).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CA - Inorganic chemistry

OECD FORD branch

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Project

<a href="/en/project/TE01020390" target="_blank" >TE01020390: Centre of the development of advanced metallic biomaterials for medical implants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Non-crystalline Solids

ISSN

0022-3093

e-ISSN

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Volume of the periodical

432

Issue of the periodical within the volume

neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

53-59

UT code for WoS article

000367769100009

EID of the result in the Scopus database

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