Biological fate of styrene oxide adducts with globin: Elimination of cleavage products in the rat urine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F16%3A43902182" target="_blank" >RIV/60461373:22310/16:43902182 - isvavai.cz</a>

Alternative codes found

RIV/75010330:_____/16:00011494

Result on the web

<a href="http://dx.doi.org/10.1016/j.toxlet.2016.08.022" target="_blank" >http://dx.doi.org/10.1016/j.toxlet.2016.08.022</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxlet.2016.08.022" target="_blank" >10.1016/j.toxlet.2016.08.022</a>

Result language

angličtina

Original language name

Biological fate of styrene oxide adducts with globin: Elimination of cleavage products in the rat urine

Original language description

The in vivo fate of globin adducts with styrene 7,8-oxide (SO), an electrophilic metabolic intermediate of styrene, was studied in male Wistar rats dosed intraperitoneally with racemic SO, 100 mg/kg b.w. Regioisomeric hydroxy(phenyl)ethyl (HPE) adducts at Cys, N-terminal Val, Lys and His in globin were determined and their elimination from blood was followed during 60 days, corresponding to life span of rat erythrocytes. In the rat urine, N?-acetylated products of hydrolytic cleavage of the HPE adducts with Cys, Lys and His were determined. On the first day post-exposure, abundant N?-acetyl-HPE-Cys adducts (mercapturic acids) formed via direct conjugation of SO with hepatic glutathione were excreted rapidly, but then a much slower phase of elimination reflecting formation of N?-acetyl-HPE-Cys via cleavage of the adducted globin was observed. A two-phase elimination occurred also in urinary N?-acetyl-HPE adducts with His and Lys. While a decline by 75?85% during the first 7 days post-exposure most likely reflected elimination of adducted albumin, the subsequent slow decline until day 60 corresponded to elimination kinetics of the adducted globin. Thus, the study not only provided original data on the fate of SO-globin adducts but also allowed to reveal general toxicokinetics properties of the urinary cleavage products as a novel type of chemical exposure biomarkers. ? 2016 Elsevier Ireland Ltd

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

—

Project

<a href="/en/project/NT13401" target="_blank" >NT13401: Degradation products of protein adducts in urine as a new type of biomarkers in toxicology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology Letters

ISSN

0378-4274

e-ISSN

—

Volume of the periodical

261

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

26-31

UT code for WoS article

000385529600003

EID of the result in the Scopus database

2-s2.0-84988700510