Cell interaction with modified nanotubes formed on titanium alloy Ti-6Al-4V
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F16%3A43902552" target="_blank" >RIV/60461373:22310/16:43902552 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/16:00459560
Result on the web
<a href="http://dx.doi.org/10.1016/j.msec.2016.04.037" target="_blank" >http://dx.doi.org/10.1016/j.msec.2016.04.037</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.msec.2016.04.037" target="_blank" >10.1016/j.msec.2016.04.037</a>
Alternative languages
Result language
angličtina
Original language name
Cell interaction with modified nanotubes formed on titanium alloy Ti-6Al-4V
Original language description
Nanotubes with diameters ranging from 40 to 60 nm were prepared by electrochemical oxidation of the Ti-6Al-4V alloy in electrolyte containing ammonium sulphate and ammonium fluoride. The nanotubes were further modified with calcium and phosphate ions or were heat treated. Polished Ti-6Al-4 V alloy served as a reference sample. The spreading of human osteoblast-like cells was similar on all nanotube samples but lower than on polished samples. The number of initially adhered cells was higher on non-modified nanotubes, but the final cell number was the highest on Ca-enriched nanotubes and the lowest on heat-treated nanotubes. However, these differences were relatively small and less pronounced than the differences in the concentration of specific molecular markers of cell adhesion and differentiation, estimated by their intensity of immunofluorescence staining. The concentration of vinculin, i.e. a protein of focal adhesion plaques, was the lowest on nanotubes modified with calcium. Collagen I, an early marker of osteogenic cell differentiation, was also the lowest on samples modified with calcium and was highest on polished samples. Alkaline phosphatase, a middle marker of osteogenic differentiation, was observed in lowest concentration on nanotubes modified with phosphorus and the highest on heat-treated samples. Osteocalcin concentrations, a late marker of osteogenic cell differentiation, were similar on all tested samples, although they tended to be the highest on heat-treated samples. Thus, osteogenic differentiation can be modulated by various additional treatments of nanotube coatings on Ti-6Al-4V implants.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JG - Metallurgy, metal materials
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials science and engineering C
ISSN
0928-4931
e-ISSN
—
Volume of the periodical
65
Issue of the periodical within the volume
August
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
313-322
UT code for WoS article
000376833200037
EID of the result in the Scopus database
—