Rapid SERS-based recognition of cell secretome on the folic acid-functionalized gold gratings

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43919490" target="_blank" >RIV/60461373:22310/19:43919490 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00216-019-01801-6" target="_blank" >https://link.springer.com/article/10.1007%2Fs00216-019-01801-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-019-01801-6" target="_blank" >10.1007/s00216-019-01801-6</a>

Result language

angličtina

Original language name

Rapid SERS-based recognition of cell secretome on the folic acid-functionalized gold gratings

Original language description

Nowadays, functionalization of the plasmon-supported nanostructured surface is considered as a powerful tool for tumour cell recognition. In this study, the SERS on a surface plasmon polariton-supported gold grating functionalized with folic acid was used to demonstrate an unpretentious recognition of melanoma-associated fibroblasts. Using cultivation media conditioned by different cells, we were able to detect reproducible differences in the secretome of melanoma-associated and normal control fibroblasts. The homogeneous distribution of plasmon energy along the grating surface was proved to provide excellent SERS signal reproducibility, while, to increase the affinity of (bio)molecules to SERS substrate, folic acid molecules were covalently grafted to the gold gratings. As proof of concept, fibroblasts were cultured in vitro, and culture media from the normal and tumour-associated lines were collected and analysed with our proposed SERS substrates. Identifying individual peaks of the Raman spectra as well as comparing their relative intensities, we showed that the proposed functional SERS platform can recognise the melanoma-associated cells without the need for further statistical spectral evaluation directly. We also demonstrated that the SERS chip created provided a stable SERS signal over a period of 90 days without loss of sensitivity. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20501 - Materials engineering

Project

<a href="/en/project/NV15-33459A" target="_blank" >NV15-33459A: Biomedical photonic devices for advanced medicinal diagnostics and therapy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

—

Volume of the periodical

411

Issue of the periodical within the volume

15

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

3309-3319

UT code for WoS article

000469757300009

EID of the result in the Scopus database

2-s2.0-85066499217