Tutorial: Guidelines for Single-Cell RT-qPCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43934022" target="_blank" >RIV/60461373:22310/21:43934022 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/21:00550587

Result on the web

<a href="https://www.mdpi.com/2073-4409/10/10/2607" target="_blank" >https://www.mdpi.com/2073-4409/10/10/2607</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells10102607" target="_blank" >10.3390/cells10102607</a>

Result language

angličtina

Original language name

Tutorial: Guidelines for Single-Cell RT-qPCR

Original language description

Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced single-cell analysis. Single-cell RT-qPCR now represents a well-established method, suitable for an efficient screening prior to single-cell RNA sequencing (scRNA-Seq) experiments, or, oppositely, for validation of hypotheses formulated from high-throughput approaches. Here, we aim to provide a comprehensive summary of the scRT-qPCR method by discussing the limitations of single-cell collection methods, describing the importance of reverse transcription, providing recommendations for the preamplification and primer design, and summarizing essential data processing steps. With the detailed protocol attached in the appendix, this tutorial provides a set of guidelines that allow any researcher to perform scRT-qPCR measurements of the highest standard.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cells

ISSN

2073-4409

e-ISSN

2073-4409

Volume of the periodical

10

Issue of the periodical within the volume

10

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

2607

UT code for WoS article

000711489100001

EID of the result in the Scopus database

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