Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F24%3A43930587" target="_blank" >RIV/60461373:22310/24:43930587 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/24:43930587

Result on the web

<a href="https://www.mdpi.com/1996-1944/17/4/915" target="_blank" >https://www.mdpi.com/1996-1944/17/4/915</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ma17040915" target="_blank" >10.3390/ma17040915</a>

Result language

angličtina

Original language name

Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials

Original language description

In vitro testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of the selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it with that of the L929 mouse fibroblast cell line. We also tested the cytotoxicity of three types of Zn-based biomaterials in two types of media. The presence of G418 used as a selection reagent decreased the sensitivity of hFOB 1.19 to Zn2+. hFOB 1.19 cell line was more sensitive to Zn2+ at elevated (restrictive) temperatures. hFOB 1.19 cell line was less sensitive to Zn2+ than L929 cell line (both as ZnCl2 and extracts of alloys). Therefore, the appropriate cultivation conditions of hFOB 1.19 during biomaterial testing should be chosen with caution.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials

ISSN

1996-1944

e-ISSN

1996-1944

Volume of the periodical

17

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

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UT code for WoS article

001172021700001

EID of the result in the Scopus database

2-s2.0-85185845123