Revealing antimicrobial resistance in stormwater with MinION
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F20%3A43921905" target="_blank" >RIV/60461373:22320/20:43921905 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S004565352031585X?token=FD44367F98BE1EAAF30C1CC22FC8E82198C6C77E98D96AE5B89C63CC9BA5FF0B67B4E6A74A7B2760B6D0BBB6C0FDFBD8&originRegion=eu-west-1&originCreation=20210505194321" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S004565352031585X?token=FD44367F98BE1EAAF30C1CC22FC8E82198C6C77E98D96AE5B89C63CC9BA5FF0B67B4E6A74A7B2760B6D0BBB6C0FDFBD8&originRegion=eu-west-1&originCreation=20210505194321</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemosphere.2020.127392" target="_blank" >10.1016/j.chemosphere.2020.127392</a>
Alternative languages
Result language
angličtina
Original language name
Revealing antimicrobial resistance in stormwater with MinION
Original language description
Discharge of urban stormwater containing organic matter, heavy metals and sometime human feces, to the natural aquatic reservoirs without any treatment is not only an environmental problem. It can lead to prevalence of antibiotic resistant bacteria in stormwater systems and transmission of antibiotic resistance genes to the environment. We performed antibiotic resistome identification and virus detection in stormwater samples from Stockholm, using publicly available metagenomic sequencing MinION data. A MinION platform offers low-cost, precise environmental metagenomics analysis. 37 groups of antibiotic resistant bacteria (ARB), 11 resistance types with 26 resistance mechanisms - antibiotic resistance genes (ARGs) giving tolerance to the aminoglycoside, beta-lactams, fosmidomycin, MLS, multidrug and vancomycin were identified using ARGpore pipeline. The majority of the identified bacteria species were related to the natural environment such as soil and were not dangerous to human. Alarmingly, human pathogenic bacteria carrying resistance to antibiotics currently used against them (Bordetella resistant to macrolides and multidrug resistant Propionibacterium avidum) were also found in the samples. Most abundant viruses identified belonged to Caudovirales and Herpesvirales and they were not carrying ARGs. Unlike the virome, resistome and ARB were not unique for stormwater sampling points. This results underline the need for extensive monitoring of the microbial community structure in the urban stormwater systems to assess antimicrobial resistance spread. (C) 2020 The Authors. Published by Elsevier Ltd.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemosphere
ISSN
0045-6535
e-ISSN
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Volume of the periodical
258
Issue of the periodical within the volume
258
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
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UT code for WoS article
000566446500113
EID of the result in the Scopus database
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