Effect of temperature on the compositions of ladderane lipids in globally surveyed anammox populations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F22%3A43923865" target="_blank" >RIV/60461373:22320/22:43923865 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/22:43923865

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0048969722018083?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0048969722018083?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2022.154715" target="_blank" >10.1016/j.scitotenv.2022.154715</a>

Result language

angličtina

Original language name

Effect of temperature on the compositions of ladderane lipids in globally surveyed anammox populations

Original language description

The adaptation of bacteria involved in anaerobic ammonium oxidation (anammox) to low temperatures will enable more efficient removal of nitrogen from sewage across seasons. At lower temperatures, bacteria typically tune the synthesis of their membrane lipids to promote membrane fluidity. However, such adaptation of anammox bacteria lipids, including unique ladderane phospholipids and especially shorter ladderanes with absent phosphatidyl headgroup, is yet to be described in detail. We investigated the membrane lipids composition (UPLC–HRMS/MS) and dominant anammox populations (16S rRNA gene amplicon sequencing, Fluorescence in situ hybridization) in 14 anammox enrichments cultivated at 10-37 °C. “Candidatus Brocadia” appeared to be the dominant organism in all but two laboratory enrichments of “Ca Scalindua” and “Ca. Kuenenia”. At lower temperatures, the membranes of all anammox populations were composed of shorter [5]-ladderane ester (reduced chain length demonstrated by decreased fraction of C20/(C18+C20)). This confirmed the previous preliminary evidence on the prominent role of this ladderane fatty acid in low-temperature adaptation. “Ca Scalindua” and “Ca. Kuenenia” had distinct profile of ladderane lipids compared to “Ca. Brocadia” biomasses with potential implications for adaptability to low temperatures. “Ca. Brocadia” membranes contained a much lower amount of C18 [5]-ladderane esters than reported in the literature for “Ca Scalindua” at similar temperature and measured here, suggesting that this could be one of the reasons for the dominance of “Ca. Scalindua” in cold marine environments. Furthermore, we propose additional and yet unreported mechanisms for low-temperature adaptation of anammox bacteria, one of which involves ladderanes with absent phosphatidyl headgroup. In sum, we deepen the understanding of cold anammox physiology by providing for the first time a consistent comparison of anammox-based communities across multiple environments.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20801 - Environmental biotechnology

Project

<a href="/en/project/GA17-25781S" target="_blank" >GA17-25781S: Physiological response of anammox bacteria to cold shocks</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Science of the Total Environment

ISSN

0048-9697

e-ISSN

—

Volume of the periodical

830

Issue of the periodical within the volume

2022

Country of publishing house

BE - BELGIUM

Number of pages

11

Pages from-to

154715

UT code for WoS article

000794872600013

EID of the result in the Scopus database

2-s2.0-85127346567