Joining the bacterial conversation: Increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F23%3A43927413" target="_blank" >RIV/60461373:22320/23:43927413 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/23:43927413

Result on the web

<a href="https://journals.asm.org/doi/10.1128/spectrum.01860-23" target="_blank" >https://journals.asm.org/doi/10.1128/spectrum.01860-23</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/spectrum.01860-23" target="_blank" >10.1128/spectrum.01860-23</a>

Result language

angličtina

Original language name

Joining the bacterial conversation: Increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Original language description

Bacteria need to be isolated in pure culture to gain access to the wide array of interesting functions they conceal. This is challenging because they do not live in isolation but instead in communities where they actively communicate and interact with each other. In this study, we aimed to increase the culturability of soil bacteria using three different signaling molecules: N-(3-oxohexanoyl)-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and 3&apos;,5&apos;-cyclic adenosine monophosphate (cAMP). The signals were added individually to soils suspended in PBS buffer to a final concentration of 5 mu M. Soil suspensions were agitated for 24 hours, after which they were serially diluted and plated on tenfold-diluted Reasoner&apos;s 2A agar that either contained or did not contain the same signaling molecule used during the extraction step. DNA was isolated from both soil suspensions and grown cultures and, after a high-throughput amplicon sequencing, differences in bacterial abundances and diversity were determined across treatments. To further explain the action of the signaling molecules on the treated soil communities, their metagenomic functions were predicted using the software PICRUSt2. N-octanoyl-L-homoserine lactone was found to increase the diversity observed on solid media, while N-(3-oxohexanoyl)-L-homoserine lactone and cAMP were not. Potentially novel isolates aided by the signaling molecules were affiliated with the genera Pseudomonas and Nocardioides.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbiology Spectrum

ISSN

2165-0497

e-ISSN

2165-0497

Volume of the periodical

11

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

"e0186023"

UT code for WoS article

001076679400001

EID of the result in the Scopus database

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