All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Joining the bacterial conversation: Increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F23%3A43927413" target="_blank" >RIV/60461373:22320/23:43927413 - isvavai.cz</a>

  • Alternative codes found

    RIV/60461373:22330/23:43927413

  • Result on the web

    <a href="https://journals.asm.org/doi/10.1128/spectrum.01860-23" target="_blank" >https://journals.asm.org/doi/10.1128/spectrum.01860-23</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1128/spectrum.01860-23" target="_blank" >10.1128/spectrum.01860-23</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Joining the bacterial conversation: Increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

  • Original language description

    Bacteria need to be isolated in pure culture to gain access to the wide array of interesting functions they conceal. This is challenging because they do not live in isolation but instead in communities where they actively communicate and interact with each other. In this study, we aimed to increase the culturability of soil bacteria using three different signaling molecules: N-(3-oxohexanoyl)-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and 3&apos;,5&apos;-cyclic adenosine monophosphate (cAMP). The signals were added individually to soils suspended in PBS buffer to a final concentration of 5 mu M. Soil suspensions were agitated for 24 hours, after which they were serially diluted and plated on tenfold-diluted Reasoner&apos;s 2A agar that either contained or did not contain the same signaling molecule used during the extraction step. DNA was isolated from both soil suspensions and grown cultures and, after a high-throughput amplicon sequencing, differences in bacterial abundances and diversity were determined across treatments. To further explain the action of the signaling molecules on the treated soil communities, their metagenomic functions were predicted using the software PICRUSt2. N-octanoyl-L-homoserine lactone was found to increase the diversity observed on solid media, while N-(3-oxohexanoyl)-L-homoserine lactone and cAMP were not. Potentially novel isolates aided by the signaling molecules were affiliated with the genera Pseudomonas and Nocardioides.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Microbiology Spectrum

  • ISSN

    2165-0497

  • e-ISSN

    2165-0497

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    "e0186023"

  • UT code for WoS article

    001076679400001

  • EID of the result in the Scopus database