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The Determination of Isoflavones and Coumestrol by Capillary Electrophoresis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F01%3A00004417" target="_blank" >RIV/60461373:22330/01:00004417 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The Determination of Isoflavones and Coumestrol by Capillary Electrophoresis

  • Original language description

    The separation of six isoflavones (biochanin A, isoformononetin, formononetin, prunetin, daidzein and genistein) and coumestrol on an uncoated fused-silica capillary electrophoresis column was optimised using alkaline borate buffer as electrolyte and DADdetection. A baseline separation of all analytes except a pair, formononetin-biochanin A was achieved at pH 10.5 in 25 minutes. Detection limits were low (0.1 mg.mL-1) and the linearity of the detector response was established in the concentration range0.4 - 60 mg.mL-1 (180 mg.mL-1 for coumestrol). Coumestrol was synthesized and the carbon signals in 13C-NMR spectrum of both coumestrol and di-O-acetylcoumestrol were assigned for the first time using two-dimensional HMQC technique.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA523%2F00%2F0567" target="_blank" >GA523/00/0567: Phytoestrogens of alfalfa as a risk factor in animal feed: occurrence, distribution, changes during ensiling</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2001

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Czech Journal of Food Science

  • ISSN

    1212-1800

  • e-ISSN

  • Volume of the periodical

    19

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    7

  • Pages from-to

    132-138

  • UT code for WoS article

  • EID of the result in the Scopus database