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Beta-galactosidase form psychrotrophic microorganism

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008936" target="_blank" >RIV/60461373:22330/03:00008936 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Beta-galactosidase form psychrotrophic microorganism

  • Original language description

    Enzymes preserving their activity under low temperatures are important for improving quality, reduction of microbial contamination and lowering the energy costs in food processing industry [1-3]. One of the straightforward applications of -galactosidaseis in technologies for production of lactose-free milk and for galactooligosacharides production. -Galactosidase (3.2.1.23), from psychrotrophic strain Arthrobacter sp.C2-2 living under extreme conditions, where the outside temperature never exceed s 5 °C, was expressed in E. coli and routinely produced in our group [4]. The crystallization experiments gave a collection of several crystal forms and sizes. Table 1. Different crystallization conditions gave different crystal habitus of ß-galaktosidase C2-2-1 from cold adapted Arthrobacter sp.C2-2: " needles 0.05 mm x 0.2 mm 10% PEG 8000, 0.1 M HEPES pH 7.5, 0.2 M Ca acetate " platelets 0.75 mm x 0.01 mm; slab shaped crystals 0.3 mm x 0.1 mm 22% PEG 4000, 0.1 M MES pH 6.5, 0.15 M (NH4)

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA204%2F02%2F0843" target="_blank" >GA204/02/0843: Complex cold adaptation study of enzymes on molecular level and their application in biotechnology</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2003

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Proceedings of Crystallography of Biological Macromolecules

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    1

  • Publisher name

    Academic Press

  • Place of publication

    Proceedings of Crystallography of Biological Mac

  • Event location

    Como

  • Event date

    Jan 1, 2003

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article