In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008957" target="_blank" >RIV/60461373:22330/03:00008957 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus
Original language description
Two types of phospholipid degrading enzymes phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl inositol specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two typesof activities; polyphosphoinositide requiring (PIP2-dependent PLD) and polyphosphoinositide independent requiring millimolar concentration of calcium (PLD?). Significantly different patterns of the activity profiles were found for soluble and membrane associated forms of all three enzymes within both processes. Membrane associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD?, while highest PI-PLC activity appears in the same stages of development of seeds and plants as forPLD?. In subcellular fractions of hypocotyls parts of young plants phospholipases were localized predominantly on plasma membranes. Biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles isola
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F00%2F1332" target="_blank" >GA522/00/1332: Role of phospholipid degrading in plant signal transduction</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
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Volume of the periodical
2003
Issue of the periodical within the volume
54
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
691-698
UT code for WoS article
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EID of the result in the Scopus database
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