Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008962" target="_blank" >RIV/60461373:22330/03:00008962 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník
Original language description
Phospholipid signalling pathway is one of the basic signalling mechanism in animal as well as plant cells. Phospholipases and phospholipids are the key components of this system. Phospholipases are divided based on the site of cleavage of the phospholipid molecules to phospholipase A2, C and D. The aim of this study was to established sophisticated method which let us to analyse products of signalling phospholipases in its entirety with one method. Phospholipid signalling pathway was studied insitu in tobacco cell suspension culture BY-2 using different types of fluorescently labelled (BODIPY) phosphatidylcholine. Incorporation of the fluorescent substrates to tobacco cell membranes was monitored by fluorescent microscopy and confirmed by pla smolysis.Fluorescent products of cleaved phosphatidylcholine were analysed by HP-TLC and newly developed HPLC methods. First the fluorescent standards of individual phospholipase products were prepared using commercial phospholipases and then use
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GA522%2F03%2F0353" target="_blank" >GA522/03/0353: Defence mechanisms in rape seed (Brassica napus) participating in plant-pathogen interaction and induced resistance</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
FEBS Advanced Course: Lipid signalling and membrane traffic
ISBN
—
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
99
Publisher name
Advanced FEBS Course
Place of publication
Itálie
Event location
Santa Mario Imbaro
Event date
Jun 20, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—