Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012835" target="_blank" >RIV/60461373:22330/04:00012835 - isvavai.cz</a>
Alternative codes found
RIV/61388980:_____/04:00106530
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings
Original language description
Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOF MS andidentification of the protein was successfully used for determination of the binder from a painting of the 19th century.
Czech name
MALDI-TOF MS jako nástroj pro identifikaci proteinů v barevných vrstvách obrazů
Czech description
MALDI-TOF MS jako nástroj pro identifikaci proteinů v barevných vrstvách obrazů

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GA203%2F04%2F2091" target="_blank" >GA203/04/2091: Material and micro-structural features in the dating and technological evaluation of the Mid-European painting</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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