Identification of isoflavonoids in Arabidopsis thaliana.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012974" target="_blank" >RIV/60461373:22330/04:00012974 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Identification of isoflavonoids in Arabidopsis thaliana.

Original language description

Extracts from leaves and inflorescence stalks of Arabidopsis thaliana and from seedlings of Lepidium sativa (Brassicaceae) were analyzed for the presence of isoflavonoids by HPLC-MS-SIM and by five isoflavonoid-specific ELISA methods after the HPLC fractionation of samples (1). Both analytical approaches revealed homologous spectra of isoflavonoids in both plant species. Formononetin and prunetin represented the main aglycones followed by biochanin A, daidzein and genistein; sissotrin was the most abundant isoflavonoid glycoside followed by ononin, daidzin and genistin. The content of individual compounds ranged from a few micrograms up to 2.2 milligrams per kilogram (dry weight). Genes homologous to enzymes involved in isoflavonoid synthesis and metabolism found in the Arabidopsis thaliana genome and their expression profiles were extracted from number of transcriptomic datasets. Genes likely to be involved in important steps of the isoflavonoid metabolism in Arabidopsis thaliana were

Czech name

Identification of isoflavonoids in Arabidopsis thaliana.

Czech description

Extracts from leaves and inflorescence stalks of Arabidopsis thaliana and from seedlings of Lepidium sativa (Brassicaceae) were analyzed for the presence of isoflavonoids by HPLC-MS-SIM and by five isoflavonoid-specific ELISA methods after the HPLC fractionation of samples (1). Both analytical approaches revealed homologous spectra of isoflavonoids in both plant species. Formononetin and prunetin represented the main aglycones followed by biochanin A, daidzein and genistein; sissotrin was the most abundant isoflavonoid glycoside followed by ononin, daidzin and genistin. The content of individual compounds ranged from a few micrograms up to 2.2 milligrams per kilogram (dry weight). Genes homologous to enzymes involved in isoflavonoid synthesis and metabolism found in the Arabidopsis thaliana genome and their expression profiles were extracted from number of transcriptomic datasets. Genes likely to be involved in important steps of the isoflavonoid metabolism in Arabidopsis thaliana were

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F03%2F0352" target="_blank" >GA525/03/0352: Isoflavonoids in the Myrtaceae and the Rutaceae families</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Chemické listy

ISBN

0009-2770

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

1022-1023

Publisher name

Česká chemická společnost

Place of publication

Praha

Event location

Nymburk

Event date

Jan 1, 2004

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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