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Molecular characterization of thermophilic and mesophilic Bacillus species-II

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00016335" target="_blank" >RIV/60461373:22330/05:00016335 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Molecular characterization of thermophilic and mesophilic Bacillus species-II

  • Original language description

    Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. Protocols for all above mentioned methods were optimised for routine laboratory use.

  • Czech name

    Charakterizace termofilních a mezofilních druhů rodu Bacillus

  • Czech description

    Metody molekulární biologie byla použity pro identifikaci termofilních a mezofilních bakterií rodu Bacillus. Bylo zkoumáno celkem 5 termofilních a 3 mezofilní izoláty a to pomocí metod: polymorfismus náhodně zmnožené DNA (RAPD), amplifikace intergenové oblasti 16S-23S rRNA (ITS-PCR) a polymorfie délky restrikčních fragmentů (RFLP) jak 16S rRNA tak produktů ITS-PCR reakce. Pro všechny výše uvedené metody byly optimalizovány jejich protokoly pro snadné laboratorní použití.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA525%2F03%2F0375" target="_blank" >GA525/03/0375: Image analyses of microbial population dynamics</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    WSEAS Transactions on Biology and Biomedicine

  • ISSN

    1109-9518

  • e-ISSN

  • Volume of the periodical

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GR - GREECE

  • Number of pages

    4

  • Pages from-to

    27-30

  • UT code for WoS article

  • EID of the result in the Scopus database