Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R).
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017788" target="_blank" >RIV/60461373:22330/06:00017788 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats(R).
Original language description
Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles, LentiKats(R). The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylene-butanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenyl-propanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was the most stable during the transformation of (R,S)-3-hydroxy-2-methylene-butanenitrile. No significant loss of the amidase activity was observed within the biocatalyst operation
Czech name
Biotransformace nitrilů Rhodococcus equi A4 imobilizovaných v LentiKats(R).
Czech description
Rhodococcus equi A4, producent nitrilhydratasové a amidasové aktivity byl imobilizován v lentiketových hydrogelových partikulích LentiKats(R). Imobilizovaný biokatalyzátor byl aplikován v biotransformaci benzonitrilu, 3-cyanopyridinu, (R,S)-3-hydroxy-2-methylene-butanenitrilu and (R,S)-3-hydroxy-2-methylene-3-phenyl-propanenitrilu. Stabilita nitrilhydratasy během opakovaného použití biokatalyzátoru byla závislá na typu substrátu.Během použití nebyla zaznamenána výrazná aktivita amidasy.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Molecular Catalysis B
ISSN
1381-1177
e-ISSN
—
Volume of the periodical
—
Issue of the periodical within the volume
39
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
3
Pages from-to
59-61
UT code for WoS article
—
EID of the result in the Scopus database
—