Optimisation of procedure for determination of polycyclic aromatic hydrocarbons and their derivatives in fish tissue: Estimation of measurements uncertainty

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017805" target="_blank" >RIV/60461373:22330/06:00017805 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Optimisation of procedure for determination of polycyclic aromatic hydrocarbons and their derivatives in fish tissue: Estimation of measurements uncertainty

Original language description

Three alternative procedures were employed for isolation of polycyclic aromatic hydrocarbons (PAHs), their methyl-derivatives and sulphur analogues from fish tissue: (i) Soxhlet extraction, (ii) batch extraction enhanced by sonication and (iii) saponification of sample followed by reextraction of analytes into hexane. Soxhlet extraction using hexane-acetone (1:1, v/v) was found as the most efficient extraction technique, with analyte recoveries in the range 70-108 %. Within optimisation of clean-up step, several types of gel permeation chromatography (GPC) systems were tested: two types of polystyrene divinylbenzene copolymer gels (PSDVB), both "soft" gel type (Bio-Beads S-X3) and "rigid" gels type (PL gel and Envirogel) in combination with various mobile phases were compared. Bio-Beads S-X3 and mobile phase chloroform were found as the most appropriate for purifying of crude extracts prior to the final determinative step. High performance liquid chromatography with fluorimetric de

Czech name

Optimalizace metody pro stanovení polycyklických aromatických uhlovodíků v rybách; odhad nejistoty metody

Czech description

Byly testovány 3 metody pro extrakci PAU z rybí tkáně (saponifikace, extrakce dle Soxhleta a sonikace). Pro přečištění extraktu byly porovnány eluční profily PAU a předpokládaných koextraktů na různých typech gelů. Pro optimalizovanou metody byly odhanuty nejistoty měření dvěma různými způsoby "top-down" a "bottom-up".

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Additives and Contaminants

ISSN

0265-203X

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

309-325

UT code for WoS article

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EID of the result in the Scopus database

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