Radioimmunoassay of 16?-Hydroxy-Dehydroepiandrosterone and its Physiological levels

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F07%3A00018983" target="_blank" >RIV/60461373:22330/07:00018983 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Radioimmunoassay of 16?-Hydroxy-Dehydroepiandrosterone and its Physiological levels
Original language description
16?-Hydroxy-dehydroepiandrosterone (16?-OH-DHEA) belongs to the products of extensive DHEA metabolism in mammalian tissues. It is a precursor of 16?-hydroxylated estrogens, increased levels of which are associated with autoimmune disorders. A highly specific radioimmunoassay of unconjugated 16?-OH-DHEA was developed and evaluated. Polyclonal rabbit antisera were raised against 3?,16?- dihydroxy-17,19-dione-19-O-(carboxymethyloxime) and 3?,16?-dihydroxy-7,17-dione-7-O-(carboxymethyloxime) BSA conjugates.Two methods were used for preparation of the conjugates. Homologous radioiodinated derivatives with tyrosine methyl ester were prepared as tracers. While antisera to 7-CMO cross-reacted with DHEA as much as by 58%, the cross-reaction of the chosen antiserum prepared via 19-oxogroup by micellar conjugation technique with 16?-OH-DHEA was only 0.13% and with all other structurally related steroids, including DHEA were lower than 0.01%. The detection limi
Czech name
Radioimmunoassay of 16?-Hydroxy-Dehydroepiandrosteronu a jeho fyziologické hladiny
Czech description
Práce popisuje vývoj imunoanalytické metody pro 16?-Hydroxy-dehydroepiandrosteron

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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