Electrophoretic determination of calystegines A3 and B2 in potato

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00020948" target="_blank" >RIV/60461373:22330/08:00020948 - isvavai.cz</a>

Alternative codes found

RIV/60109807:_____/08:#0000085

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electrophoretic determination of calystegines A3 and B2 in potato

Original language description

Potatoes, members of the Solanaceae plant family, contain calystegines, water-soluble nortropane alkaloids, which are biologically active as glycosidase inhibitors. The content of calystegines A3 and B2 in different varieties of potato and in various parts of the tubers (whole potato, peel, flesh, and sprouts) were analysed by new capillary zone electrophoresis and capillary isotachophoresis methods and by the routine GC method. The optimised background electrolyte for capillary zone electrophoretic analysis was mixture of 20 mM histidine, 20 mM N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid and 20% (v/v) methanol in demineralized water. Calystegines were detected by indirect UV detection at 210 nm. A clear separation of calystegines from other components of the methanolic sample extract was achieved within 4 minutes. The electrolytes for isotachophoretic analysis consisted of 5 mM NH4OH, 10 mM N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, 0.1% hydroxyethylcellulose and 20% (

Czech name

Elektroforetické stanovení kalysteginu A3 a B2 v bramborech

Czech description

Potatoes, members of the Solanaceae plant family, contain calystegines, water-soluble nortropane alkaloids, which are biologically active as glycosidase inhibitors. The content of calystegines A3 and B2 in different varieties of potato and in various parts of the tubers (whole potato, peel, flesh, and sprouts) were analysed by new capillary zone electrophoresis and capillary isotachophoresis methods and by the routine GC method. The optimised background electrolyte for capillary zone electrophoretic analysis was mixture of 20 mM histidine, 20 mM N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid and 20% (v/v) methanol in demineralized water. Calystegines were detected by indirect UV detection at 210 nm. A clear separation of calystegines from other components of the methanolic sample extract was achieved within 4 minutes. The electrolytes for isotachophoretic analysis consisted of 5 mM NH4OH, 10 mM N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, 0.1% hydroxyethylcellulose and 20% (

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Volume of the periodical

1181

Issue of the periodical within the volume

1-2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

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UT code for WoS article

000253506600016

EID of the result in the Scopus database

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