Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021070" target="_blank" >RIV/60461373:22330/08:00021070 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage

Original language description

Reactive oxygen species (ROS), e.g., hydrogen peroxide, are aerobic cellular by products, where excess oxidatively damages DNA, proteins and lipids. This is of particular concern regarding carcinogenesis. The Fenton reaction generates ROS. Several reports suggest haemoglobin-containing heme-iron, like other iron-rich substances, produce ROS in vitro due to this catalyst. As haemoglobin has been considered a villain, playing a negative role for human health in vitro, this potentially damaging effect on cellular DNA requires clarification in vivo. Here, we show that under the coexistence of haemoglobin derivatives and heme with hydrogen peroxide, DNA did not damage. This inhibition was confirmed in cell culture using a single cell gel electrophoresis assay, comet assay, proving haemoglobin has catalase activity. These results dispute prior findings that in vitro haemoglobin generates ROS, which damages DNA-inducing carcinogenesis. In fact, haemoglobin delays or inhibits carcinogenesis ca

Czech name

Vyhodnocení poškození DNA vyvolané reakcí kyslíku a inhibiční efekt hemových bílkoviny na toto poškození

Czech description

Reakční formy kyslíku (ROS), např. peroxid vodíku jsou aerobní buněčné produkty, jejichž přebytek oxidateivně poškozuje DNA, bílkoviny a lipidy. Bylo prokázáno, že při současné koexistenci hemových barviv a peroxidu vodíku DNA není poškozena. Tato inhibice byla potvrzena použitímpoužitím jednoduché gelové elektroforézy, comet assay, dokazující, že haemoglobin má katalázovou aktivitu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fleischwirtschaft International

ISSN

0179-2415

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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