Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021213" target="_blank" >RIV/60461373:22330/08:00021213 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/08:00310140

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8

Original language description

The complete 98,192bp nucleotide sequence was determined for plasmid pA81, which is harbored by the haloaromatic acid-degrading bacterium Achromobacter xylosoxidans A8. The majority of the 103 open reading frames identified on pA81 could be categorized as either "backbone" genes, genes encoding (halo)aromatic compound degradation, or heavy metal resistance determinants. The backbone genes controlled conjugative transfer, replication and plasmid stability, and were well conserved with other IncP1-beta plasmids. Genes encoding (halo)aromatic degradation were clustered within a type I transposon, TnAxI, and included two ring-hydroxylating oxygenases (ortho-halobenzoate oxygenase, salicylate 5-hydroxylase) and a modified ortho-cleavage pathway for chlorocatechol degradation. The cluster of heavy metal resistance determinants was contained within a Type II transposon TnAxII, and included a predicted P-type ATPase and cation diffusion facilitator system. Genes identical to those carried by T

Czech name

Nukleotidová sekvence, organizace a charakterizace plasmidu pA81 Achromobacter xylosoxidans A8 kódujícího geny katabolismu haloaromátů

Czech description

Byla stanovena kompletní 98 192 bp sekvence plasmidu pA81 bakterie Achromobacter xylosoxidans A8. Na tomto plasmidu byly identifikovány geny kódující enzymy zodpovědné za katabolismus haloaromatických látek. Bylo identifikováno 103 otevřených čtecích rámců kódujících proteiny zodpovědné buď za degradaci haloaromátů nebo hrajících roli při resistenci bakterie proti těžkým kovům. Všechny nalezené geny byly asociovány s mobilními elementy, které umožňují přenos takovýchto genů mezi bakteriemi.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/1M0520" target="_blank" >1M0520: Center for Applied Genomics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research in Microbiology

ISSN

0923-2508

e-ISSN

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Volume of the periodical

159

Issue of the periodical within the volume

2

Country of publishing house

BE - BELGIUM

Number of pages

10

Pages from-to

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UT code for WoS article

000254807300006

EID of the result in the Scopus database

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