Caleosin of Arabidopsis thaliana: Effect of Calcium on Functional and Structural Properties

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021235" target="_blank" >RIV/60461373:22330/08:00021235 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Caleosin of Arabidopsis thaliana: Effect of Calcium on Functional and Structural Properties
Original language description
A non-radioactive blot binding assay has proved the capacity of a purified recombinant form of Arabidopsis thaliana caleosin (AtClo1), a key protein of this plant oil body, to bind calcium. Calcium affected recombinant caleosin aggregation state, solubility, and electrophoretic mobility on SDS-PAGE. The effect of calcium on interfacial behavior of recombinant caleosin was studied at three interfaces: air/water (A/W), purified oil/water (O/W), and air/phosholipid/water (A/PLs/W). Recombinant caleosin wasable to decrease interfacial tension (IFT) at A/W and O/W interfaces as a function of concentration and calcium, whereas no interaction was detected at the A/PLs/W interface. Effect of calcium was time dependent, and its amplitude strongly varied with the interface considered. Reconstituted oil bodies were used to prove the involvement of recombinant caleosin in their calcium-driven aggregation and coalescence. Calcium ions at concentration as low as 100 nM were able to strongly modify
Czech name
Caleosin z Arabidopsis thaliana: vliv vápníku na funkci a strukturu
Czech description
Byl studován vliv vápníku na funkci a strukturu Caleosinu z Arabidopsis thaliana:

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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