Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021237" target="_blank" >RIV/60461373:22330/08:00021237 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture

Original language description

The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, thespecific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the hig

Czech name

Produkce rekombinantních proteinů buňkami Picha pastoris Mut+ rostoucími v kontinuální kultuře

Czech description

Kinetika růstu, nadprodukce rekombinantního proteinu a fyziologie kvasinek Pichia pastoris Mut+ byly sledována na směsném substrátu obsahujícím 2 zdroje uhlíku (glukosu a methanol) rostoucí v kontinuální kultuře. Specificá rychlost spotřeby methanolu vzrůstala se zvyšující se zřeďovací rychlostí, maximum 0.090.01 g.gCDW-1.h-1 bylo dosaženo při D = 0.15 h-1; oba ko-substraty byly utilizovány současně a zcela. Maximální koncentrace trypsinogenu 145 mg.L-1 a maximální specifická productivita (1.17 mg.gCDW-1.h-1) byly dosaženy při D = 0.13 h-1.

Type

D - Article in proceedings

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/1P05OE189" target="_blank" >1P05OE189: Large-scale implementation of an optimum technology for high-productivity processes with recombinant methylotrophic yeasts</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 13th International Biotechnology Symposium and Exhibition

ISBN

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ISSN

0168-1656

e-ISSN

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Number of pages

2

Pages from-to

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Publisher name

Dalian Institute of Chemical Physics

Place of publication

Dalian

Event location

Dalian, China

Event date

Oct 12, 2008

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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