Determination of Lamotrigine by Isotachophoresis - Capillary Zone Electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F09%3A00022426" target="_blank" >RIV/60461373:22330/09:00022426 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

čeština

Original language name

Stanovení lamotriginu v séru pomocí on-line spojení kapilární isotachoforesy a kapilární zónové elektroforesy

Original language description

The aim of this work was to develop and validate electrophoretic methods for the determination of a new antiepileptic, lamotrigine (LAM). We tested the conditions for analysis of LAM in blood serum by two capillary isotachophoreses (ITP-ITP) and by capilary isotachophoresis - capillary zone electrophoresis (ITP-CZE). Best results were obtained using the ITP-CZE method with UV detection at 254 nm. The latter method offers a simple and rapid procedure for the determination of LAM in blood serum. A low sample volume is highly convenient especially for the determination in children. The analysis takes some 20 min. The linearity of the method was proved in the range 0-20 mg LAM/I. The limits of detection and quantification were 0.25 and 0.83 mg LAM/l, respectively. The variation coefficients were below 10% at three concentration levels (1, 7 and 20 mg l(-1)). The method was compared with HPLC using the Passing-Bablok regression analysis; no significant differences were found.

Czech name

Stanovení lamotriginu v séru pomocí on-line spojení kapilární isotachoforesy a kapilární zónové elektroforesy

Czech description

The aim of this work was to develop and validate electrophoretic methods for the determination of a new antiepileptic, lamotrigine (LAM). We tested the conditions for analysis of LAM in blood serum by two capillary isotachophoreses (ITP-ITP) and by capilary isotachophoresis - capillary zone electrophoresis (ITP-CZE). Best results were obtained using the ITP-CZE method with UV detection at 254 nm. The latter method offers a simple and rapid procedure for the determination of LAM in blood serum. A low sample volume is highly convenient especially for the determination in children. The analysis takes some 20 min. The linearity of the method was proved in the range 0-20 mg LAM/I. The limits of detection and quantification were 0.25 and 0.83 mg LAM/l, respectively. The variation coefficients were below 10% at three concentration levels (1, 7 and 20 mg l(-1)). The method was compared with HPLC using the Passing-Bablok regression analysis; no significant differences were found.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

—

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemické listy

ISSN

0009-2770

e-ISSN

—

Volume of the periodical

103

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

—

UT code for WoS article

000263745600008

EID of the result in the Scopus database

—