Strip-based immunoassay for rapid detection of thiabendazole.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024229" target="_blank" >RIV/60461373:22330/10:00024229 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Strip-based immunoassay for rapid detection of thiabendazole.

Original language description

This study reports development of an immunostrip test for thiabendazole detection based on indirect competitive principle using carbon particles as a label. Nitrocellulose membrane strip was coated with a thiabendazole-protein conjugate in the defined test zone. In flow of an antibody-carbon complex and thiabendazole along the strip, the intensity of black colour formed in the test line reflected the thiabendazole concentration and semi-quantitative estimation could be carried out visually. The optimized test was accomplished within 10 min and the visual detection limit was achieved 0.25 ngmL-1 of standard sample. Moreover, immunostrip was evaluated quantitatively using scanning densitometry. Based on standard curve, the detection limit of the proposedtest was as low as 0.08?0.03 ngmL-1 with an IC50 value of 0.60?0.08 ngmL-1 and a linear working range of 0.11?4.13 ngmL-1. Results of testing precision, stability, and specificity demonstrated that the assay provided a reliable performan

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA525%2F07%2F0618" target="_blank" >GA525/07/0618: Immunoassay of pesticides in baby food and fruit juices - development of rapid easy-to-use detection methods</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biosensors and Bioelectronics

ISSN

0956-5663

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

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UT code for WoS article

000277930000020

EID of the result in the Scopus database

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