A novel approach for the determination of membrane protein expression levels based on LC-MS/MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43891994" target="_blank" >RIV/60461373:22330/11:43891994 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/11:00365269 RIV/60076658:12310/11:43883064
Result on the web
<a href="http://dx.doi.org/10.1080/10826076.2011.569808" target="_blank" >http://dx.doi.org/10.1080/10826076.2011.569808</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/10826076.2011.569808" target="_blank" >10.1080/10826076.2011.569808</a>
Alternative languages
Result language
angličtina
Original language name
A novel approach for the determination of membrane protein expression levels based on LC-MS/MS
Original language description
Membrane proteins play crucial roles in a number of cellular processes. Thus, the determination of their expression levels under various physiological conditions is crucial in many biological and biochemical studies. However, the extremely hydrophobic nature of proteins possessing transmembrane domains complicates their analysis. Therefore, we developed a new approach toward quantification of membrane proteins in complex mixtures combining reversed-phase chromatography on C4 resin and mass spectrometrictechniques using iTRAQ (Isobaric tags for relative and absolute quantitation) labels. Reversed-phase chromatography on C4 resin with stepwise elution with 2-propanol was used to reduce contamination with hydrophilic proteins and to fractionate membraneproteins according to their hydrophobicity. The method was tested on an artificially prepared defined ratio of plasma membrane proteins obtained from cell cultures of Arabidopsis thaliana by 2-phase partitioning of microsomal fractions. T
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Liquid Chromatography and Related Technologies
ISSN
1082-6076
e-ISSN
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Volume of the periodical
34
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1004-1013
UT code for WoS article
000291807100005
EID of the result in the Scopus database
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