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Identification of regioisomers and enantiomers of triacylglycerols in different yeasts

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43895126" target="_blank" >RIV/60461373:22330/13:43895126 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1002/jssc.201300657" target="_blank" >http://dx.doi.org/10.1002/jssc.201300657</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/jssc.201300657" target="_blank" >10.1002/jssc.201300657</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Identification of regioisomers and enantiomers of triacylglycerols in different yeasts

  • Original language description

    LC with atmospheric pressure chemical ionization (APCI) MS with reversed and chiral phases was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon and Yarrowia. Chiral LC?APCI-MS is based on using two columns in series packed with a 3,5- dimethylphenyl carbamate modified ?-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated. Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were OOO (triolein), sn-OPoO (oleopalmitoleoolein), and sn-PoPoO (dipalmitoleoolein). In strains producing palmitoleicacid in amounts above 25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10 to 25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strai

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP503%2F11%2F0215" target="_blank" >GAP503/11/0215: Analysis of the composition of triacylglycerols and other lipids in nutritionally important microbial oils and fats.</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Separation Science

  • ISSN

    1615-9306

  • e-ISSN

  • Volume of the periodical

    36

  • Issue of the periodical within the volume

    říjen 2013

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    22

  • Pages from-to

    3310-3320

  • UT code for WoS article

  • EID of the result in the Scopus database