Cost-effective method for the preparation of uniformly labeled myristoylated proteins for NMR measurements

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897513" target="_blank" >RIV/60461373:22330/14:43897513 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22810/14:43897513

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S1046592814000552#" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1046592814000552#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pep.2014.03.005" target="_blank" >10.1016/j.pep.2014.03.005</a>

Result language

angličtina

Original language name

Cost-effective method for the preparation of uniformly labeled myristoylated proteins for NMR measurements

Original language description

Nuclear magnetic resonance (NMR) is a powerful technique for solving protein structures or studying their interactions. However, it requires molecules labeled with NMR sensitive isotopes like carbon C-13 and nitrogen N-15. The recombinant expression of labeled proteins is simple to perform but requires quite expensive chemicals. When there is a need for special labeled chemicals, like uniformly C-13-labeled myristic acid, the price significantly rises. Here we describe a cost-effective method for the recombinant expression of uniformly labeled myristoylated proteins in Escherichia coli demonstrated on the production of Mason-Pfizer monkey virus matrix protein. We used the ability of E. coli to naturally synthetize myristic acid. When grown in isotopically labeled medium the myristic acid will be labelled as well. Bacteria were co-transfected with plasmid carrying gene for yeast N-myristoyltransferase which ensures myristoylation of expressed protein. This process provided 1.8 mg of the

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP302%2F12%2F1895" target="_blank" >GAP302/12/1895: Role of retroviral matrix protein in virus particle targeting and interaction with plasma membrane.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protein Expression and Purification

ISSN

1046-5928

e-ISSN

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Volume of the periodical

99

Issue of the periodical within the volume

červenec 2014

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

6-9

UT code for WoS article

000336870800002

EID of the result in the Scopus database

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