Tracing of nutritional aspects in human blood serum by UHPLC-Qorbitrap MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43901503" target="_blank" >RIV/60461373:22330/15:43901503 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Tracing of nutritional aspects in human blood serum by UHPLC-Qorbitrap MS
Original language description
Statins belong to the class of hypolipidemic drugs mainly used to treat high levels of cholesterol in blood. They act as competitive inhibitors of a HMG-CoA reductase, enzyme limiting the rate of cholesterol synthesis pathway. This inhibition not only leads to depletion of cholesterol and its esters with fatty acids, but also to depletion of intermediates of this metabolic pathway (farnesyl- and geranylgeranyl pyrophosphate), which can play important role in tumor proliferation. Knowledge of concentrations of all these compounds may thus serve for better assessment of the overall effect of statins on treated patients. The main analytical challenge of the study was to increase the method throughput by integrating of all the mentioned analytes into the one determinative step. This was definitely not an easy task because of different physico-chemical properties of all compounds of interest (8 semipolar statins, and their biologically active metabolites, 2 polar intermediates of cholesterol synthesis, and relatively non-polar cholesterol and its 3 main esters with fatty acids). Various ways of sample preparation were examined in order to minimize the content of the blood serum proteins, and assure good recoveries and low matrix effect for these analytes. The separation by ultra-high performance liquid chromatography coupled with tandem high resolution mass spectrometry (UHPLC-HRMS/MS) method with Q-orbitrap as mass analyzer was performed. The potential of Q-orbitrap mass spectrometry, i.e. the sensitivity and the broad dynamic range, were fully exploited for analysis of these heterogeneous com pounds differing also in their natural concentration in blood, thus in demands on the analytical performance. The troubleshooting we met during the method development, as well as the final performance characteristics, will be discussed in details.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT13112" target="_blank" >NT13112: Studies of anticancer effects of statins</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů